|
KMID : 0624620160490030191
|
|
BMB Reports
2016 Volume.49 No. 3 p.191 ~ p.196
|
|
|
Loss of phospholipase D2 impairs VEGF-induced angiogenesis
|
|
Lee Chang-Sup
Ghim Jae-Wang
Song Park-Yong
Suh Pann-Ghill
Ryu Sung-Ho
|
|
|
Abstract
|
|
|
|
Vascular endothelial growth factor (VEGF) is a key mediator of angiogenesis and critical for normal embryonic development and repair of pathophysiological conditions in adults. Although phospholipase D (PLD) activity has been implicated in angiogenic processes, its role in VEGF signaling during angiogenesis in mammals is unclear. Here, we found that silencing of PLD2 by siRNA blocked VEGF-mediated signaling in immortalized human umbilical vein endothelial cells (iHUVECs). Also, VEGF-induced endothelial cell survival, proliferation, migration, and tube formation were inhibited by PLD2 silencing. Furthermore, while Pld2-knockout mice exhibited normal development, loss of PLD2 inhibited VEGF-mediated ex vivo angiogenesis. These findings suggest that PLD2 functions as a key mediator in the VEGF-mediated angiogenic functions of endothelial cells.
|
|
|
KEYWORD
|
|
|
Angiogenesis, Aorta ring, Endothelial cells, Phospholipase D, Tube formation, VEGF
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|